TAGMe DNA Methylation Detection Kits (qPCR) mo te mate pukupuku Cervical / Endometrial Cancer
WHAKAMAHI HUA
Kore urutomo
E tika ana ma te paraihe pukupuku me nga tauira paninga Pap.
Watea
Ko te hangarau rapunga methylation Me-qPCR taketake ka taea te whakaoti i tetahi taahiraa i roto i nga haora 3 kaore he huringa bisulfite.
Moata
Ka kitea i te wa o mua i te mate pukupuku.
Aunoatanga
I te taha o te raupaparorohiko tātari hua kua whakaritea, ko te whakamaoritanga o nga hua he mea aunoa me te panui tika.
Nga tauari tono
Te tirotiro moata
Tangata hauora
Te Aromatawai morearea mo te mate pukupuku
Taupori morearea nui (he pai mo te papillomavirus tangata morearea nui (hrHPV) he pai ranei mo te cytology exfoliation cervical / pai mo te papillomavirus tangata morearea nui (hrHPV) he pai ranei mo te cytology exfoliation cervical)
Te Aroturuki Whakahoki
Taupori matapae
WHAKAMAHI WHAKAMAHI
Ka whakamahia tenei kete mo te in vitro te kitenga o te hypermethylation o te ira PCDHGB7 i roto i nga tauira o te kopu.Mo te mate pukupuku mate pukupuku, he tohu pai te hua ka nui ake te mate o te karaehe 2, teitei ake ranei te neoplasia intraepithelial cervical (CIN2+, tae atu ki te CIN2, CIN3, adenocarcinoma in situ, me te mate pukupuku cervical), e hiahia ana kia nui ake te colposcopy me te tirotiro histopathological. .Engari, ko nga hua whakamatautau kino e tohu ana he iti te tupono o te CIN2+, engari kaore e taea te whakakore katoa.Ko te tātaritanga whakamutunga me whai i runga i te colposcopy me/ranei nga kitenga histopathological.I tua atu, mo te mate pukupuku endometrial, ko te hua pai e tohu ana he nui ake te tupono o te mate pukupuku endometrial me te mate pukupuku, e hiahia ana ki te tirotiro histopathological o te endometrium.Engari, ko nga hua whakamatautau kino e tohu ana he iti te tupono o te mate pukupuku o mua me te mate pukupuku endometrial, engari kaore e taea te whakakore katoa.Ko te tātaritanga whakamutunga me hangai i runga i nga hua whakamatautau histopathological o te endometrium.
Ko te PCDHGB7 he mema o te whanau protocadherin γ kāhui ira.Kua kitea te Protocadherin ki te whakahaere i nga tikanga koiora penei i te tipu o te pūtau, te huringa pūtau, te apoptosis, te whakaekenga, te heke me te autophagy o nga pūtau puku i roto i nga momo tohu tohu, me te wahangu o te ira na te hypermethylation o te rohe kaiwhakatairanga e tata ana ki te tupu me te whanaketanga. o nga mate pukupuku maha.Kua korerohia ko te hypermethylation o te PCDHGB7 e hono ana ki nga momo pukupuku, penei i te lymphoma kore-Hodgkin, te mate pukupuku o te uma, te mate pukupuku pukupuku, te mate pukupuku endometrial me te pukupuku pukupuku.
MĀTĀPONO WHARE
Kei roto i tenei kete te reagent tangohanga waikawa karihi me te reagent detection PCR.Ka tangohia te waikawa nucleic ma te tikanga autō-bead-based.I ahu mai tenei kete i runga i te maataapono o te tikanga PCR ine rewharewha, ma te whakamahi i te tauhohenga PCR methylation-motuhake ki te tātari i te DNA tauira, me te kite i nga waahi CpG o te ira PCDHGB7 me te tohu mana kounga o roto i nga kongakonga ira tohutoro G1 me G2.Ko te taumata methylation o PCDHGB7 i roto i te tauira, te uara Me ranei, ka tatauhia i runga i te PCDHGB7 ira methylated DNA amplification Ct uara me te uara Ct o te tohutoro.Ko te PCDHGB7 ira hypermethylation pai, kino ranei ka whakatauhia i runga i te uara Me.
