TAGMe DNA Methylation Detection Kits (qPCR) mo te mate pukupuku Urothelial
WHAKAMAHI HUA
Tikanga
Kua whakamanahia i runga i te 3500 tauira haumanu i roto i nga rangahau-matapo-rua-pokapū, ko te hua he 92.7% te motuhake me te 82.1%.
Watea
Ko te hangarau rapunga methylation Me-qPCR taketake ka taea te whakaoti i tetahi taahiraa i roto i nga haora 3 kaore he huringa bisulfite.
Kore urutomo
Ko te 30 mL anake o te tauira mimi e hiahiatia ana kia kitea nga momo mate pukupuku e 3, tae atu ki te mate pukupuku renal pelvis, mate pukupuku ureteral, mate pukupuku pukupuku i te wa ano.
Nga tauari tono
Te Tohu Awhina
Te taupori e mate ana i te hematuria kore mamae/ e whakapaetia ana he urothelial (mate pukupuku ureteral/mate pukupuku renal pelvis)
Te Aromatawai morearea mo te mate pukupuku
He pokanga/ chemotherapy-e hiahia ana te taupori me te mate pukupuku urothelial;
Te Aroturuki Whakahoki
Taupori i muri i te mahi me te mate pukupuku urothelial
WHAKAMAHI WHAKAMAHI
Ka whakamahia tenei kete mo te rapunga kounga in vitro mo te hypermethylation o te ira Urothelial Carcinoma(UC) i roto i nga tauira urothelial.Ko te hua pai e tohu ana he nui ake te mate o te UC, e hiahia ana ki te tirotiro i te cystoscope me/ranei te tirotiro histopathological.Engari, ko nga hua whakamatautau kino e tohu ana he iti te tupono o te UC, engari kaore e taea te whakakore katoa.Ko te tātaritanga whakamutunga me whai i runga i te cystoscope me/ranei nga kitenga histopathological.
MĀTĀPONO WHARE
Kei roto i tenei kete te reagent tangohanga waikawa karihi me te reagent detection PCR.Ka tangohia te waikawa nucleic ma te tikanga autō-bead-based.I ahu mai tenei kete i runga i te maataapono o te tikanga PCR ine rewharewha, ma te whakamahi i te tauhohenga PCR methylation-motuhake hei tātari i te DNA tauira, ka kitea i te wa kotahi nga waahi CpG o te ira UC me te tohu mana kounga o roto i nga kongakonga ira tohutoro G1 me G2.Ko te taumata methylation o te ira UC, e kiia ana ko te uara Me, ka tatauhia i runga i te ira UC methylated DNA amplification Ct uara me te uara Ct o te tohutoro.Ko te UC ira hypermethylation mana pai, kino ranei ka whakatauhia i runga i te uara Me.
Nga Kete Tirohanga Methylation DNA (qPCR) mo te mate pukupuku Urothelial
| Te tono haumanu | Te tātaritanga awhina haumanu o te cnacer urothelial;te aromatawai i te kaha o te maimoatanga pokanga/chemotheray;te aroturuki i te hokinga mai i muri i te mahi |
| Ira kimi | UC |
| Momo tauira | Tauira pūtau kua werohia te mimi (parataiao mimi) |
| Tikanga whakamatautau | Hangarau PCR ine korikori |
| Nga tauira e tika ana | ABI7500 |
| Whakatakotoranga kete | 48 nga whakamatautau / kete |
| Nga Tikanga Rokiroki | Me penapena te kete A ki te 2-30 ℃ Me penapena te kete B ki te -20±5 ℃ Ka whai mana mo te 12 marama. |
